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mouse ace2 sirna  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse ace2 sirna
    AT2R activation increases UCP1 and CITED1 expressions in white adipocytes. ( a and b ) Mouse white adipose cells (day 4) were transfected with control siRNAs, <t>ACE2-siRNA,</t> AT1R-siRNA or AT2R-siRNAs for 2–3 days, before treated without (−) (control) or with (+) AngII, ZD7155 or PD123319 for another 4 days. ( c ) Mouse white adipocytes were treated without (−) (control) or with (+) 100 nM M024/C21, 100 nM CGP42112 or PD123319 for 4 days. The representative immunoblots of protein expressions (UCP1, CITED1, ACE2, AT1R, AT2R, PPARγ, PRDM16, aP2 and actin), and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.
    Mouse Ace2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ace2+sirna/pmc05661636-47-29-43?v=Santa+Cruz+Biotechnology
    Average 94 stars, based on 11 article reviews
    mouse ace2 sirna - by Bioz Stars, 2026-07
    94/100 stars

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    1) Product Images from "Angiotensin type 2 receptor activation promotes browning of white adipose tissue and brown adipogenesis"

    Article Title: Angiotensin type 2 receptor activation promotes browning of white adipose tissue and brown adipogenesis

    Journal: Signal Transduction and Targeted Therapy

    doi: 10.1038/sigtrans.2017.22

    AT2R activation increases UCP1 and CITED1 expressions in white adipocytes. ( a and b ) Mouse white adipose cells (day 4) were transfected with control siRNAs, ACE2-siRNA, AT1R-siRNA or AT2R-siRNAs for 2–3 days, before treated without (−) (control) or with (+) AngII, ZD7155 or PD123319 for another 4 days. ( c ) Mouse white adipocytes were treated without (−) (control) or with (+) 100 nM M024/C21, 100 nM CGP42112 or PD123319 for 4 days. The representative immunoblots of protein expressions (UCP1, CITED1, ACE2, AT1R, AT2R, PPARγ, PRDM16, aP2 and actin), and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.
    Figure Legend Snippet: AT2R activation increases UCP1 and CITED1 expressions in white adipocytes. ( a and b ) Mouse white adipose cells (day 4) were transfected with control siRNAs, ACE2-siRNA, AT1R-siRNA or AT2R-siRNAs for 2–3 days, before treated without (−) (control) or with (+) AngII, ZD7155 or PD123319 for another 4 days. ( c ) Mouse white adipocytes were treated without (−) (control) or with (+) 100 nM M024/C21, 100 nM CGP42112 or PD123319 for 4 days. The representative immunoblots of protein expressions (UCP1, CITED1, ACE2, AT1R, AT2R, PPARγ, PRDM16, aP2 and actin), and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.

    Techniques Used: Activation Assay, Transfection, Control, Western Blot

    Signaling pathways underlying the AngII–AT2R-induced browning of white adipocytes. ( a – c ) Time course of the phosphorylation and total protein levels of ERK1/2, Akt and AMPK in mouse white adipocytes, without (−) (control) or with (+) exposure to AngII and ZD7155. Top panels, representative immunoblots; bottom panels, statistics (mean±s.e.m., n =3) of the optical density ratio between pERK and ERK, pAkt and Akt, or pAMPK and AMPK. ( d and e ) Mouse white adipose cells (day 4) were transfected with control siRNA, ERK1/2-siRNAs, Akt-siRNA or AMPK-siRNAs for 2–3 days before treated without (−) (control) or with (+) AngII and ZD7155 for 4 days. The representative immunoblots and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.
    Figure Legend Snippet: Signaling pathways underlying the AngII–AT2R-induced browning of white adipocytes. ( a – c ) Time course of the phosphorylation and total protein levels of ERK1/2, Akt and AMPK in mouse white adipocytes, without (−) (control) or with (+) exposure to AngII and ZD7155. Top panels, representative immunoblots; bottom panels, statistics (mean±s.e.m., n =3) of the optical density ratio between pERK and ERK, pAkt and Akt, or pAMPK and AMPK. ( d and e ) Mouse white adipose cells (day 4) were transfected with control siRNA, ERK1/2-siRNAs, Akt-siRNA or AMPK-siRNAs for 2–3 days before treated without (−) (control) or with (+) AngII and ZD7155 for 4 days. The representative immunoblots and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.

    Techniques Used: Protein-Protein interactions, Phospho-proteomics, Control, Western Blot, Transfection



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    mouse ace2 sirna  (Santa Cruz Biotechnology)
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    AT2R activation increases UCP1 and CITED1 expressions in white adipocytes. ( a and b ) Mouse white adipose cells (day 4) were transfected with control siRNAs, <t>ACE2-siRNA,</t> AT1R-siRNA or AT2R-siRNAs for 2–3 days, before treated without (−) (control) or with (+) AngII, ZD7155 or PD123319 for another 4 days. ( c ) Mouse white adipocytes were treated without (−) (control) or with (+) 100 nM M024/C21, 100 nM CGP42112 or PD123319 for 4 days. The representative immunoblots of protein expressions (UCP1, CITED1, ACE2, AT1R, AT2R, PPARγ, PRDM16, aP2 and actin), and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.
    Mouse Ace2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ace2+sirna/pmc05661636-47-29-43?v=Santa+Cruz+Biotechnology
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    mouse at 1 sirna  (Santa Cruz Biotechnology)
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    AT2R activation increases UCP1 and CITED1 expressions in white adipocytes. ( a and b ) Mouse white adipose cells (day 4) were transfected with control siRNAs, <t>ACE2-siRNA,</t> AT1R-siRNA or AT2R-siRNAs for 2–3 days, before treated without (−) (control) or with (+) AngII, ZD7155 or PD123319 for another 4 days. ( c ) Mouse white adipocytes were treated without (−) (control) or with (+) 100 nM M024/C21, 100 nM CGP42112 or PD123319 for 4 days. The representative immunoblots of protein expressions (UCP1, CITED1, ACE2, AT1R, AT2R, PPARγ, PRDM16, aP2 and actin), and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.
    Mouse At 1 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    AT2R activation increases UCP1 and CITED1 expressions in white adipocytes. ( a and b ) Mouse white adipose cells (day 4) were transfected with control siRNAs, ACE2-siRNA, AT1R-siRNA or AT2R-siRNAs for 2–3 days, before treated without (−) (control) or with (+) AngII, ZD7155 or PD123319 for another 4 days. ( c ) Mouse white adipocytes were treated without (−) (control) or with (+) 100 nM M024/C21, 100 nM CGP42112 or PD123319 for 4 days. The representative immunoblots of protein expressions (UCP1, CITED1, ACE2, AT1R, AT2R, PPARγ, PRDM16, aP2 and actin), and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Angiotensin type 2 receptor activation promotes browning of white adipose tissue and brown adipogenesis

    doi: 10.1038/sigtrans.2017.22

    Figure Lengend Snippet: AT2R activation increases UCP1 and CITED1 expressions in white adipocytes. ( a and b ) Mouse white adipose cells (day 4) were transfected with control siRNAs, ACE2-siRNA, AT1R-siRNA or AT2R-siRNAs for 2–3 days, before treated without (−) (control) or with (+) AngII, ZD7155 or PD123319 for another 4 days. ( c ) Mouse white adipocytes were treated without (−) (control) or with (+) 100 nM M024/C21, 100 nM CGP42112 or PD123319 for 4 days. The representative immunoblots of protein expressions (UCP1, CITED1, ACE2, AT1R, AT2R, PPARγ, PRDM16, aP2 and actin), and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.

    Article Snippet: For gene silencing of ACE2 ( ACE2 ), AT1R ( AGTR1 ) or AT2R ( AGTR2 ), adipose cells (day 4 after induction of differentiation) were transfected with a mouse ACE2 siRNA (sc-41401), AT1R siRNA (sc-29751), AT2R siRNA (sc-29753) or control siRNA (sc-37007) (Santa Cruz Biotechnology) as previously described., Specifically, adipose cells were incubated with Opti-MEM containing a complex of Lipofectamine-RNAiMAX transfection reagent (0.5% (v/v), Life Technologies, ThermoFisher Scientific) with siRNAs (25 n m ) for 4 h, followed by the addition of grown medium and incubated for another 2–3 days.

    Techniques: Activation Assay, Transfection, Control, Western Blot

    Signaling pathways underlying the AngII–AT2R-induced browning of white adipocytes. ( a – c ) Time course of the phosphorylation and total protein levels of ERK1/2, Akt and AMPK in mouse white adipocytes, without (−) (control) or with (+) exposure to AngII and ZD7155. Top panels, representative immunoblots; bottom panels, statistics (mean±s.e.m., n =3) of the optical density ratio between pERK and ERK, pAkt and Akt, or pAMPK and AMPK. ( d and e ) Mouse white adipose cells (day 4) were transfected with control siRNA, ERK1/2-siRNAs, Akt-siRNA or AMPK-siRNAs for 2–3 days before treated without (−) (control) or with (+) AngII and ZD7155 for 4 days. The representative immunoblots and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.

    Journal: Signal Transduction and Targeted Therapy

    Article Title: Angiotensin type 2 receptor activation promotes browning of white adipose tissue and brown adipogenesis

    doi: 10.1038/sigtrans.2017.22

    Figure Lengend Snippet: Signaling pathways underlying the AngII–AT2R-induced browning of white adipocytes. ( a – c ) Time course of the phosphorylation and total protein levels of ERK1/2, Akt and AMPK in mouse white adipocytes, without (−) (control) or with (+) exposure to AngII and ZD7155. Top panels, representative immunoblots; bottom panels, statistics (mean±s.e.m., n =3) of the optical density ratio between pERK and ERK, pAkt and Akt, or pAMPK and AMPK. ( d and e ) Mouse white adipose cells (day 4) were transfected with control siRNA, ERK1/2-siRNAs, Akt-siRNA or AMPK-siRNAs for 2–3 days before treated without (−) (control) or with (+) AngII and ZD7155 for 4 days. The representative immunoblots and the statistics (mean±s.e.m., n =4; normalized to actin densities) are shown accordingly. * P <0.05, ** P <0.01, *** P <0.001 versus control; # P <0.05 and ## P <0.01 between indicated pairs.

    Article Snippet: For gene silencing of ACE2 ( ACE2 ), AT1R ( AGTR1 ) or AT2R ( AGTR2 ), adipose cells (day 4 after induction of differentiation) were transfected with a mouse ACE2 siRNA (sc-41401), AT1R siRNA (sc-29751), AT2R siRNA (sc-29753) or control siRNA (sc-37007) (Santa Cruz Biotechnology) as previously described., Specifically, adipose cells were incubated with Opti-MEM containing a complex of Lipofectamine-RNAiMAX transfection reagent (0.5% (v/v), Life Technologies, ThermoFisher Scientific) with siRNAs (25 n m ) for 4 h, followed by the addition of grown medium and incubated for another 2–3 days.

    Techniques: Protein-Protein interactions, Phospho-proteomics, Control, Western Blot, Transfection